Female Rat Preputial Glands as a Source of β-Glucuronidase
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چکیده
منابع مشابه
Physical and chemical properties of beta-glucuronidase from the preputial gland of the female rat.
In order to obtain sufficient quantities of beta-glucuronidase for use in structural studies, the enzyme was purified from its richest known source, the female rat preputial gland, by a method similar to that of Ohtsuka and Wakabayashi (1969) (Enzymologia 12, 109). The purified enzyme has an S-o20, w of 12.5 S and a D-o20, w of 4.3 times 10- minus 7 cm-2 S-minus 1. Sedimentation diffusion and s...
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In order to obtain sufficient quantities of P-glucuronidase for use in structural studies, the enzyme was purified from its richest known source, the female rat preputial gland, by a method similar to that of Ohtsuka and Wakabayashi (1969) (Enzymologia 12, 109). The purified enzyme has an s&,,~ of 12.5 S and a D&., of 4.3 x lo-’ cm* SC’. Sedimentation diffusion and sedimentation equilibrium yie...
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Messenger RNA was isolated from rat preputial glands by guanidine HCl extraction, ethanol and salt precipitation, followed by chromatography on oligo(dT) cellulose. Double-stranded cDNA was synthesized from the mRNA and inserted into the Pst 1 site of the plasmid pBR322 by the poly(dG).poly(dC) tailing and annealing procedure. The hybrid plasmids were used to transform E. coli HBlOl. Recombinan...
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چکیده ندارد.
15 صفحه اولProliferative and nonproliferative lesions of the rat and mouse mammary, Zymbal's, preputial, and clitoral glands.
The mammary gland of laboratory rodents is an important organ for the evaluation of effects of xenobiotics, especially those that perturb hormonal homeostasis or are potentially carcinogenic. Mammary gland cancer is a leading cause of human mortality and morbidity worldwide and is a subject of major research efforts utilizing rodent models. Zymbal's, preputial, and clitoral glands are standard ...
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ژورنال
عنوان ژورنال: Nature
سال: 1961
ISSN: 0028-0836,1476-4687
DOI: 10.1038/192359a0